Data Entry Maintenance

Metadata Commons Identifier

HMC000100

Project Title

Integrative Molecular and Functional Analysis of Human Sperm Subpopulations to Identify New Biomarkers of Fertilization Potential

Project Description

<div class="ck-content"><p class="p1" style="-webkit-text-stroke-width:0px;color:black;font-family:Times, serif;font-size:7.5pt;font-style:normal;font-variant-caps:normal;font-weight:400;letter-spacing:normal;margin:0cm;orphans:auto;text-align:justify;text-decoration:none;text-indent:0px;text-transform:none;white-space:normal;widows:auto;word-spacing:0px;"><span style="font-family:Calibri, sans-serif;font-size:12pt;">Human ejaculates are composed of sperm subsets with heterogeneous characteristics. Comparative studies of sperm subpopulations with differences in motility may serve to investigate the functional and molecular features that are crucial for reaching </span><span style="color:rgb(0,0,0);"><span style="-webkit-text-stroke-width:0px;caret-color:rgb(0, 0, 0);font-family:Calibri, sans-serif;font-size:12pt;font-style:normal;font-variant-caps:normal;font-weight:400;letter-spacing:normal;orphans:auto;text-align:start;text-decoration:none;text-indent:0px;text-transform:none;white-space:normal;widows:auto;word-spacing:0px;" lang="ES">the oocyte and fertilizing. The main objective of this project is to identify functional and molecular markers that characterize sperm subpopulations with high and low motility. Semen samples from 11 donors with proven fertility were processed by density gradient centrifugation to isolate high (F1) and low (F2) motility sperm subpopulations. Besides motility, we evaluated viability, chromatin integrity, mitochondrial membrane potential, capacitation, and acrosomal status. F1 and F2 were subjected to comparative methylome and transcriptome analyses by whole-genome bisulfite sequencing and RNA sequencing, respectively. Further validation of candidate biomarkers at the RNA and protein levels was performed with semen samples from six normozoospermic volunteers using quantitative PCR and Western blotting.</span></span></p><ul><li>Metadata accessions at the Epigenome Reference Registry (EpiRR) for RNA-seq and WGBS from both sperm samples subpopulations for each donor<ul><li>donor #1: <a href="https://www.ebi.ac.uk/epirr/epigenomes/IHECRE00003820">https://www.ebi.ac.uk/epirr/epigenomes/IHECRE00003820 </a><a href="https://www.ebi.ac.uk/epirr/epigenomes/IHECRE00003823">https://www.ebi.ac.uk/epirr/epigenomes/IHECRE00003823</a></li><li>donor #2: <a href="https://www.ebi.ac.uk/epirr/epigenomes/IHECRE00003831">https://www.ebi.ac.uk/epirr/epigenomes/IHECRE00003831</a> <a href="https://www.ebi.ac.uk/epirr/epigenomes/IHECRE00003840">https://www.ebi.ac.uk/epirr/epigenomes/IHECRE00003840</a></li></ul></li></ul></div>

Project Funder(s)

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán, Fundación Tambre, Cátedra Salvador Zubirán, Universidad Nacional Autónoma de México, FunSaEd A.C.

Project Institution(s)

Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán

Project Investigator(s)

Martin Hirst, Pedro Caballero-Campo, Saúl Lira-Albarrán, Alexandra Amaral, Chibo Hong, Nakul Shah, Annaick Carles, Daofeng Li, David Barrera, Gabriel Hernández-Silva, Joao Ramalho-Santos, Ting Wang, Fernando Larrea, Joe Costello, Paolo Rinaudo, Mayel Chirinos

Data Access Request URL*

https://ega-archive.org/datasets/EGAD00001003963

Keywords

Sperm, Sperm motility, DNA methylation, RNA-seq, Sperm function, Fertilization Potential.

Publication Link

https://pubmed.ncbi.nlm.nih.gov/40168948/

Study Completed

Post to REACH BC Platform

Cohort Name

Human Sperm Subpopulations Study

Cohort Size

Disease/Condition Studied

male infertility

Enrollment Time Window

Biobanking Consent Available

Medical History Available

Ethnicity Availability

Time Course

Patient Phenotypes

Patient Outcomes

Clinical Data Types Available

Time Course Data Points

Enrollment City

Groups

Project

Cohort

Enrollment City

Groups

Samples and Omics