Data Entry Maintenance

Metadata Commons Identifier

HMC000078

Project Title

Epigenetic Profiling of Human Normal Hematopoietic Stem Cells from Umbilical Cord Blood

Project Description

<p>Full epigenome profiling of primary cell populations FACS purified from pooled normal human umbilical cord blood samples:</p> <ul> <li>Cord Blood progenitors (markers: CD34+CD38-)</li> <li>Common Myeloid Progenitors (CMPs)</li> <li>Granulocyte Monocyte Progenitors (GMPs)</li> <li>Megakaryocyte-Erythroid Progenitors (MEPs)</li> <li>Monocytes</li> <li>Erythroid progenitors</li> </ul> <p>Assays completed:  RNA-seq, DNA methylation (PBAL - full genome methylation analysis), native ChIP-seq for the following histone marks: H3K4me3, H3K4me1, H3K9me3, H3K27ac, H2K27me3, H3K36me3, and input control.</p> <p><a href="https://www.ebi.ac.uk/epirr">Accession numbers at Epigenome Reference Registry (EpiRR)</a> </p> <ul> <li>CB_CD34+CD38-: IHECRE00003837</li> <li>CMP: IHECRE00003826</li> <li>GMP: IHECRE00003838</li> <li>MEP: IHECRE00003825</li> <li>Monocytes: IHECRE00003848</li> <li>Erythroid: IHECRE00003834</li> </ul> <p>Reference epigenomes were generated as part of the International Human Epigenomics Consortium (<a href="https://ihec-epigenomes.org/">IHEC</a>)</p> <section data-type="supplementary-materials" id="app-2"></section>

Project Funder(s)

Genome British Columbia, Canadian Institutes of Health Research (CIHR), Terry Fox Research Institute

Project Institution(s)

University of British Columbia

Project Investigator(s)

Martin Hirst, Connie Eaves

Data Access Request URL*

https://ega-archive.org/datasets/EGAD00001004950

Keywords

umbilical cord blood, fetal blood, primary cells, stem cells, epigenomics, bivalent promoter, nucleosome density

Publication Link

https://pubmed.ncbi.nlm.nih.gov/27851972/

Study Completed

Cohort Name

Umbilical Cord Blood Purified Cells

Cohort Size

Disease/Condition Studied

normal

Enrollment Time Window

Biobanking Consent Available

Medical History Available

Ethnicity Availability

Time Course

Patient Phenotypes

Patient Outcomes

Clinical Data Types Available

Time Course Data Points

Enrollment City

Groups

Project

Cohort

Enrollment City

Groups

Samples and Omics