Data Entry Maintenance

Metadata Commons Identifier

HMC000063

Project Title

Epigenomic Programming in Early Fetal Brain Development

Project Description

<div title="Page 35" class="page"> <div class="layoutArea"> <div class="column"> <p>To explore epigenetic landscape of the developing human brain, dissected brain tissue and primary neurospheres derived from cortex and ganglionic eminence (GE) were obtained from four phenotypically normal human fetuses (gestational weeks (GW): GW13, GW15 and GW17 - the two samples at GW17 are monozygotic twins). Immunohistochemistry analysis of primary neurospheres cultures showed cell-type specificity with cortex derived neurospheres displaying an increased neuronal phenotype compared to GE derived neurospheres. To confirm the progenitor status of the neurosphere cultures, they were differentiated into neuroblasts (DCX+, CALRETININ+, TUJ1+) and astrocytes (GFAP+).</p> <div title="Page 35" class="page"> <div class="layoutArea"> <div class="column"> <p>Massively parallel sequencing based assays were employed to generate chromatin immunoprecipitation sequencing (ChIP-seq), whole genome bisulfite sequencing (WGBS), methylated DNA immunoprecipitation sequencing (MeDIP-seq), methylation sensitive restriction enzyme sequencing (MRE-seq), mRNA-sequencing (RNA-seq) and microRNA-sequencing (miRNA-seq) datasets from the dissected primary brain tissue and the neurospheres derived from cortex and GE</p> <p>These data provide a comprehensive set of epigenomic features for further exploration of the epigenetic state changes associated with normal fetal brain development and its dysfunction in disease.</p> </div> </div> </div> <p>(NPC: Neural Progenitor Cells)</p> <p><a href="http://www.ncbi.nlm.nih.gov/geo/roadmap/epigenomics/">http://www.ncbi.nlm.nih.gov/geo/roadmap/epigenomics/</a> (links for raw sequencing data repositories)</p> </div> </div> </div>

Project Funder(s)

Genome British Columbia, Canadian Institutes of Health Research (CIHR), Canadian Epigenetics, Environment and Health Research Consortium (CEEHRC), NIH

Project Institution(s)

University of British Columbia, BC Cancer Research Institute

Project Investigator(s)

Martin Hirst, Torsten Nielsen

Data Access Request URL*

Keywords

fetal brain, twins, epigenomics, epigenetic, neurospheres

Publication Link

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7857341/

Study Completed

Cohort Name

Epigenomic Programming in Early Fetal Brain Development

Cohort Size

Disease/Condition Studied

healthy

Enrollment Time Window

Biobanking Consent Available

Medical History Available

Ethnicity Availability

Time Course

Patient Phenotypes

phenotypically normal human fetuses

Patient Outcomes

Clinical Data Types Available

sex, gestational weeks (GW)

Time Course Data Points

Enrollment City

Groups

Project

Cohort

Enrollment City

Groups

Samples and Omics