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Metadata Commons Identifier

HMC000053

Project Title

HEARTBiT: A Multi-Marker Blood Test for Acute Cardiac Transplant Rejection

Project Description

Heart transplant patients must undergo tissue biopsies of their heart so that they can be monitored for acute rejection, which is when their body rejects their new implanted heart. Tissue biopsies can be painful and are costly. The goal of this study is to develop a new blood test, called HEARTBiT, that can be used to monitor for acute rejection. Heart transplant patients who consent to participate in the study will have their blood and data collected each time they have a biopsy. Their blood and data will be analyzed for markers such as genes and proteins to see if the HEARTBiT test performs well. In the past 10 years, through the Biomarkers in Transplantation Study (BiT), we used unbiased 'omics' profiling approaches to uncover blood-based mRNA (gene expression) and proteins that monitor treatable acute rejection. We used Affymetrix microarrays to analyze whole blood RNA, and mass spectrometry to analyze plasma proteins. This work was done on 66 heart transplant patients enrolled at SPH and was then extended to 84 additional patients from 8 Canadian transplant sites. The technology-agnostic biomarker pipeline the PROOF Centre team has established has been successfully applied to develop biomarkers for transplant rejection. The objectives of the study were: •Evaluate the performance of HEARTBiT, a custom 9-mRNA biomarker test developed on the NanoString platform, in an environment suitable for clinical translation, on up to 4000 newly collected samples from 400 heart transplant patients across North America. •Examine the HEARTBiT score on samples collected serially in the first year post-transplant to identify predictive signatures of rejection and characterize underlying biology. •Develop and assess 5 promising protein biomarker candidates on NanoString, test 7 candidate miRNAs, and evaluate panels combining RNA, miRNA, and protein to improve HEARTBiT.   <p style="box-sizing: border-box; outline-color: var(--ut-focus-outline-color,-webkit-focus-ring-color); font-size: 14px; line-height: inherit; margin-block: 0px; color: rgb(38, 38, 38); font-family: system-ui, -apple-system, 'system-ui', 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Fira Sans', 'Droid Sans', 'Helvetica Neue', sans-serif; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;" class="MsoNormal">  <p style="box-sizing: border-box; outline-color: var(--ut-focus-outline-color,-webkit-focus-ring-color); font-size: 14px; line-height: inherit; margin-block: 0px; color: rgb(38, 38, 38); font-family: system-ui, -apple-system, 'system-ui', 'Segoe UI', Roboto, Oxygen, Ubuntu, Cantarell, 'Fira Sans', 'Droid Sans', 'Helvetica Neue', sans-serif; font-style: normal; font-variant-ligatures: normal; font-variant-caps: normal; font-weight: 400; letter-spacing: normal; orphans: 2; text-align: start; text-indent: 0px; text-transform: none; widows: 2; word-spacing: 0px; -webkit-text-stroke-width: 0px; white-space: normal; background-color: rgb(255, 255, 255); text-decoration-thickness: initial; text-decoration-style: initial; text-decoration-color: initial;" class="MsoNormal">

Project Funder(s)

Canadian Institutes of Health Research (CIHR)

Project Institution(s)

University of British Columbia, PROOF Centre of Excellence

Project Investigator(s)

Scott Tebbutt, Bruce McManus, Raymond Ng

Data Access Request URL*

Keywords

heart transplantation, acute cellular rejection, monitoring, biomarkers

Publication Link

Study Completed

Cohort Name

HEARTBiT

Cohort Size

166

Disease/Condition Studied

heart transplant

Enrollment Time Window

Biobanking Consent Available

Medical History Available

Ethnicity Availability

Time Course

Patient Phenotypes

acute cellular rejection, no rejection

Patient Outcomes

infections, graft loss, death

Clinical Data Types Available

laboratory results, blood immunossupressive levels, immunossupression, rejection treatment

Time Course Data Points

Pre-transplant and at weeks 2, 3, 4, 6, 8, 10, 12, 16, 18, 20, 24, 32, year 1 post-transplant, and at the time of suspected rejection.

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Cohort

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Groups

Samples and Omics